Comparative Evaluation of Tissue Protein Separations Applying One- Dimensional Gel Electrophoresis and Capillary Gel Electrophoresis

Comparative Evaluation of Tissue Protein Separations Applying One- Dimensional Gel Electrophoresis and Capillary Gel Electrophoresis

The objective of this study was to characterize and compare the water and salt soluble proteins from rat liver and muscle by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and sodium dodecyl sulphate polymer-filled capillary gel electrophoresis (SDS-CGE). The evaluation and comparison of separations realized with the use of both techniques was done. It was found tha...

Gel Electrophoresis in Capillary Electrophoresis

The discovery that antigen}antibody interaction could be produced not only in liquids, but also on gel media, such as agar or agarose gels, with the formation of insoluble immunoprecipitates, opened the door to the development of the gel diffusion techniques for immunoprecipitation analysis. The Rrst of these techniques, known as double diffusion, was introduced by Ouchterlony in 1948. In this ...

Capillary Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Gel Electrophoresis

Electrophoresis is the migration of charged particles or molecules in an electric field. This occurs when the substances are in aqueous solution. The speed of migration is dependent on the applied electric field strength and the charges of the molecules. Thus, differently charged molecules will form individual zones while they migrate. In order to keep diffusion of the zones to a minimum, elect...